Westerheide Lab
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Research

Research in our laboratory  is directed at understanding the molecular importance of the heat shock  response (HSR) on cellular metabolism, aging and  disease.  The HSR is a universally conserved pathway that allows cells to recover from protein damage induced by heat stress, heavy metals, normal development, and disease states such as neurodegenerative disease and cancer. The  heat shock transcription factor HSF1, the master regulator of the HSR, transcriptionally activates molecular chaperones and other genes that are essential for cell survival.  We have found that HSF1 is positively regulated by the longevity factor SIRT1.  SIRT1 is a metabolically  regulated deacetylase that is required for full HSF1 transcription. In  collaboration with Stan Stevens in the department, our work was the first to  show that HSF1 is acetylated within its DNA binding domain at lysine 80, and  that acetylation at this site disrupts DNA binding. Deacetylation of this  residue by SIRT1 maintains HSF1 in a DNA-binding competent state, activating  HSF1-dependent transcription and the HSR.   Our work suggests that HSF1 and SIRT1 together compose a molecular network  that connects cell metabolism, stress and lifespan.

Research Projects:

Examine the  effect of cellular metabolism and aging on the HSR
The  identification of SIRT1 as a positive regulator of HSF1 transcription suggests that the HSR is likely controlled by  metabolism during the aging process.  To investigate the effects of aging  and metabolism on the HSR, experiments are underway using low and high passage  human fibroblasts to determine whether replicative lifespan alters the 
HSR-responsiveness in a SIRT1-dependent manner.   Distinct metabolic pathways regulated by HSF1 and SIRT1 will be  evaluated to determine how these two important proteins modulate cell fate  networks in response to the HSR and aging.

Further  define the mechanism of SIRT1 regulation of the HSR
Although we know that SIRT1 activity is required to regulate HSF1 transcription, the molecular mechanisms by
which SIRT1 regulates HSF1 during HSR are not fully  understood.  As SIRT1 mRNA and protein levels do not change during the  HSR, my laboratory is examining whether SIRT1 activity is post-translationally  regulated by stress.  We are testing  whether HSR-inducing stressors alter SIRT1 cellular localization, activity  and/or interaction with HSF1 during heat shock and metabolic stress. The goal of this project is to identify novel  mechanisms by which SIRT1 activity is modulated by heat shock and other  stressors.   
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Analyze the  interplay of PTMs on HSF1 function
HSF1 is a highly post-translationally modified  protein that is hyperphosphorylated and SUMOylated.  Working together with Stan Stevens, we have  found that HSF1 is also hyperacetylated on 9 lysine resides following heat  shock.  Given that HSF1 is an essential  transcription factor in controlling cell fate in response to multiple stress  responses, it is likely that the various post-translational modifications of  HSF1 generate a “code” that fine-tunes the HSR.  We are unraveling the  post-translational code of HSF1 using mass spectrometry and site-directed mutagenesis to determine the importance of these modifications.  These  studies will provide insight into how HSF1 activation is differentially regulated according to the stress signals, and will potentially allow determination of how HSF1 controls stress-specific gene expression in a cell-type specific manner.

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